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Zuivering van Biotinylated Cell Surface Proteins uit
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Zuivering van Biotinylated Cell Surface Proteins uit

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

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Een gemodificeerde dichtheidscentrifugatiegradient gebaseerde methodologie werd gebruikt om epitheelcellen van Rhipicephalus microplus gutweefsel te isoleren. Oppervlakte gebonden eiwitten werden biotinyleerd en gezuiverd door streptavidine magnetische kralen voor gebruik in downstream toepassingen.

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Biochemie nummer 125 celoppervlakte-eiwitten biotinylatie teken microplus epitheelcellen van de darm
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