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Journal / Genetics / Protokoll för C-Brick-DNA-standardmontering med…
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JoVE Journal Genetics
Protokoll för C-Brick-DNA-standardmontering med användning av Cpfl
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Protokoll för C-Brick-DNA-standardmontering med användning av Cpfl

Article DOI: 10.3791/55775-v • 12:03 min • June 15th, 2017
June 15th, 2017 •
1, 1, 1
1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

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CRISPR-associerat protein Cpf1 kan styras av ett speciellt konstruerat CRISPR-RNA (crRNA) för att klyva dubbelsträngat DNA vid önskade ställen, vilket genererar klibbiga ändar. Baserat på denna egenskap etablerades en DNA-monteringsstandard (C-Brick), och ett protokoll som beskriver dess användning beskrivs här.

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Genetics Syntetisk biologi CRISPR Cpf1 C-Brick klibbiga ändar DNA-montering, Monteringsstandard
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