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Journal / Genetics / Cpf1'i Kullanarak C-Brick DNA Standart…
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Genetics

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JoVE Journal Genetics
Cpf1'i Kullanarak C-Brick DNA Standart Montajı İçin Protokoller
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Cpf1'i Kullanarak C-Brick DNA Standart Montajı İçin Protokoller

Article DOI: 10.3791/55775-v • 12:03 min • June 15th, 2017
June 15th, 2017 •
1, 1, 1
1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

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CRISPR ile ilişkili protein Cpf1, özel olarak tasarlanmış bir CRISPR RNA (crRNA) yardımıyla, istenilen noktalarda çift sarmallı DNA'yı parçalayıp yapışkan uçlar üretebilir. Bu karakteristiğe dayanarak, bir DNA montaj standardı (C-Brick) oluşturuldu ve kullanımını ayrıntılandıran bir protokol burada açıklandı.

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Genetik Sayı 124 sentetik biyoloji CRISPR Cpf1 C-Tuğla yapışkan uçlar DNA montajı, Montaj standardı
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