Journal
/
Genetics
/
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

DOI:
10.3791/57320-v

•

10:16 min

•

June 28, 2018

•

, , , ,
1Nuclear Dynamics Programme, The Babraham Institute,Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol,Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute,Babraham Research Campus, 5Department of Biological Science,Florida State University

Chapters

  • 00:04Title
  • 00:52Formaldehyde Fixation
  • 02:14HindIII Digestion and Biotinylation of Restriction Fragment Overhangs
  • 04:09Hybrid In-solution Capture
  • 06:53Isolation of Promoter Fragment-containing Ligation Products
  • 08:55Results: IL8 PCHi-C Profile in Human Hematopoietic Cells
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

DNA regulatory elements, such as enhancers, control gene expression by physically contacting target gene promoters, often through long-range chromosomal interactions spanning large genomic distances. Promoter Capture Hi-C (PCHi-C) identifies significant interactions between promoters and distal regions, enabling the assignment of potential regulatory sequences to their target genes.

Tags

Keywords: Promoter Capture Hi-CGenome-wide ProfilingPromoter InteractionsGene Regulatory ElementsTarget GenesGenome StructureGene Expression ControlNoncoding Disease VariantsRegulatory Control SequencesCell LysisRestriction BufferCell Nuclei

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57320?article_type=v

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
check_url/57320?article_type=v

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
check_url/57320?article_type=v

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
check_url/57320?article_type=v

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
check_url/57320?article_type=v

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
check_url/57320?article_type=v

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
check_url/57320?article_type=v

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
check_url/57320?article_type=v

High-Resolution Comparison of Bacterial Conjugation Frequencies
check_url/57320?article_type=v

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
check_url/57320?article_type=v

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Read Article