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Immunology and Infection

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JoVE Journal Immunology and Infection
Induktion af celledifferentiering og single cell imaging af
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Induktion af celledifferentiering og single cell imaging af

Article DOI: 10.3791/55842-v • 08:38 min • May 15th, 2017
May 15th, 2017 •
1, 1, 1
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

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Denne protokol muliggør enkeltcellemikroskopi af de forskelligt adskilte Vibrio parahaemolyticus svømmer- og sværmerceller. Fremgangsmåden frembringer en population af swarmerceller, der er let tilgængelige til enkeltcelleanalyse og dækker fremstilling af cellekulturer, induktion af swarmer differentiering, prøvepræparation og billedanalyse.

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Immunology , Fluorescensmikroskopi enkeltcelleanalyse differentiering swarming svømning protein lokalisering
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