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Inspelning synaptisk plasticitet i akut Hippocampus skivor underhålls i en små volymer återvinning - Perfusion- och nedsänkning-typ kammaresystem
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
DOI:

09:51 min

January 01, 2018

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Chapters

  • 00:05Title
  • 01:05Preparation of Acute Hippocampal Slices
  • 02:21Modifications of the Carbogenation for the aCSF Recycling of Small Reservoirs
  • 03:08Recording Synaptic Responses in a Submersion Slice Chamber
  • 07:28Results: Representative Recordings of Activity-dependent Potentiaation of Synaptic Transmission Using Outflow-carbogenation and Reservoir-carbogenation
  • 08:08Conclusion

Summary

Automatic Translation

Det här protokollet beskriver stabiliseringen av syrehalten i en liten volym av återvunnet buffert och metodologiska aspekter av inspelning aktivitet-beroende synaptisk plasticitet i nedsänkt akut Hippocampus skivor.

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