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JoVE Journal Developmental Biology
Reprogrammierung primäre Fruchtwasser und Membrane Zellen zur Pluripotenz in Xeno-freien Bedingungen
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Reprogrammierung primäre Fruchtwasser und Membrane Zellen zur Pluripotenz in Xeno-freien Bedingungen

Article DOI: 10.3791/56003-v • 09:34 min • November 27th, 2017
November 27th, 2017 •
1, 2, 1, 3, 3,4,5, 1, 1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

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Dieses Protokoll beschreibt die Reprogrammierung von primären amniotische Flüssigkeit und Membran mesenchymalen Stammzellen in induzierte pluripotente Stammzellen unter Verwendung eines nicht-Integration von episomal Ansatzes in vollem Umfang chemisch definierten Bedingungen. Verfahren der Gewinnung, Kultur, Neuprogrammierung und Charakterisierung der daraus resultierenden induzierten pluripotenten Stammzellen durch strenge Methoden werden detailliert beschrieben.

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Entwicklungsbiologie Ausgabe 129 iPSC Fruchtwasser Amnion Neuprogrammierung episomal E8 Mittel vitronectin
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