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JoVE Journal Neuroscience
Toxicidade do Amyloid-β42 de modelagem e neurodegeneração no cérebro adulto de Zebrafish
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Toxicidade do Amyloid-β42 de modelagem e neurodegeneração no cérebro adulto de Zebrafish

Article DOI: 10.3791/56014-v • 10:01 min • October 25th, 2017
October 25th, 2017 •
1, 2, 1, 1, 3, 2, 1,3
1German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association, 2B CUBE, Center for Molecular Bioengineering, Technische Universität Dresden, 3Center for Regenerative Therapies Dresden (CRTD), TU Dresden

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Este protocolo descreve a síntese, caracterização e injeção de peptídeos monomérico amiloide-β42 para gerar toxicidade amiloide no zebrafish adulto para estabelecer um modelo de doença de Alzheimer, seguido de análise histológica e detecção de agregações.

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Neurociência edição 128 β42 Amyloid zebrafish neurodegeneração microinjeção de cerebroventricular doença de Alzheimer síntese do peptide de fase sólida
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