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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

リアルタイムで開発を可視化する多光子タイムラプスイメージング

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Journal (Developmental Biology)

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Journal (Developmental Biology)

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

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リアルタイムで開発を可視化する多光子タイムラプスイメージング: ゼブラフィッシュ胚の神経堤細胞の可視化

Article DOI: 10.3791/56214-v • 10:13 min • August 9th, 2017

August 9th, 2017 •

Antionette L. Williams¹, Brenda L. Bohnsack¹

¹Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan

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長い波長多光子蛍光励起顕微鏡レーザーの高度な光学技術の組み合わせは、Tg (sox10:EGFP) および Tg (foxd3:GFP) ゼブラフィッシュ胚の神経堤移行の高解像度、リアルタイム三次元画像をキャプチャする実装されました。

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発生生物学、問題 126、多光子蛍光タイムラプスイメージング、レーザーのスキャン顕微鏡検査、前眼部、 sox10、 foxd3、先天性眼疾患、神経の頂上、目の開発、ゼブラフィッシュ

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