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Developmental Biology
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多光子时间推移成像实时可视化发展: 迁移神经嵴细胞在斑马鱼胚胎中的可视化
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
多光子时间推移成像实时可视化发展: 迁移神经嵴细胞在斑马鱼胚胎中的可视化
DOI:
10.3791/56214-v
•
10:13 min
•
August 09, 2017
•
Antionette L. Williams
,
Brenda L. Bohnsack
1
Department of Ophthalmology and Visual Sciences, Kellogg Eye Center
,
University of Michigan
Chapters
00:05
Title
00:48
Embryo Collection for Time-lapse Imaging
01:51
Embryo Preparation
02:52
Preparation of 2% Low-melt Agarose and Mounting the Embryo
04:34
Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
07:26
Post-acquisition Processing
08:10
Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
09:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
激光扫描与波长多光子荧光显微镜的先进光学技术组合的实施是为了捕获高分辨率的、 三维的、 实时成像中甘油三酯 (
sox10:EGFP
) 和甘油三酯 (
foxd3:GFP
) 斑马鱼胚胎神经嵴迁移。
Tags
Multi-photon Time Lapse Imaging
Zebrafish Embryos
Neural Crest Cell Migration
Developmental Biology
Cell Migration Visualization
Confocal Microscopy
Multi-photon Laser
Embryo Collection
Transgenic Embryos
GFP
PTU
LowMelt Agarose
Open Bath Chamber
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