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Journal / Genetics / 一种从任意 DNA 源进行大规模 gRNA…
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Genetics

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JoVE Journal Genetics
一种从任意 DNA 源进行大规模 gRNA 库生产的通用协议
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一种从任意 DNA 源进行大规模 gRNA 库生产的通用协议

Article DOI: 10.3791/56264-v • 10:32 min • December 6th, 2017
December 6th, 2017 •
1, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian-Universität, BioMedical Center, 2Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health

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生成大型 gRNA 库的方法应简单、高效且具有成本效益。我们描述了一种基于目标 DNA 酶消化的 gRNA 库的生成协议。这种方法, CORALINA (全面 gRNA 图书馆一代通过控制核酸活动) 提出了一个替代昂贵的定制寡核苷酸合成。

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遗传学 问题 130 gRNA 图书馆 CRISPR Cas9 核酸 图书馆世代 全基因组
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