Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

This content is Open Access.

一种从任意 DNA 源进行大规模 gRNA 库生产的通用协议
 
Click here for the English version

一种从任意 DNA 源进行大规模 gRNA 库生产的通用协议

Article DOI: 10.3791/56264
December 6th, 2017

Chapters

Summary December 6th, 2017

Please note that all translations are automatically generated.

Click here for the English version.

生成大型 gRNA 库的方法应简单、高效且具有成本效益。我们描述了一种基于目标 DNA 酶消化的 gRNA 库的生成协议。这种方法, CORALINA (全面 gRNA 图书馆一代通过控制核酸活动) 提出了一个替代昂贵的定制寡核苷酸合成。

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter