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Genetics
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植物标本标本的鲁棒 DNA 分离与高通量测序库建设
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Genetics
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Please note that all translations are automatically generated.
Click here for the English version.
植物标本标本的鲁棒 DNA 分离与高通量测序库建设
DOI:
10.3791/56837-v
•
13:03 min
•
March 08, 2018
•
Saman Saeidi*
1
,
Michael R. McKain*
1,2
,
Elizabeth A. Kellogg
1
Donald Danforth Plant Science Center
,
2
Department of Biological Sciences
,
The University of Alabama
Chapters
00:04
Title
00:58
DNA Extraction
04:39
DNA Shearing
05:28
Bead Clean-up
07:06
Library Preparation
07:40
Adaptor Ligation
08:46
PCR Amplification
09:12
Size Selection for Desired Library Size and Quality Control
11:04
Results: Isolation and High-throughput Sequencing of Poor Quality DNA Isolated from Various Herbarium Species
12:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文阐述了从植物标本室材料中提取 dna 隔离和高通量测序库的详细协议, 包括抢救异常质量较差的 dna。
Tags
DNA Isolation
High-throughput Sequencing
Herbarium Specimens
Phylogenomic Studies
CTAB Extraction
RNase A
Phenol-chloroform Purification
Library Construction
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