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透射电镜下体的样品制备与成像

JoVE Journal
Biology

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JoVE Journal Biology

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

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透射电镜下体的样品制备与成像

37,411 Views

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11:15 min

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January 04, 2018

DOI:

10.3791/56482-v

DOI:

10.3791/56482-v

•

11:15 min

January 04, 2018

•

39 Views

Min Kyo Jung¹, Ji Young Mun²

¹Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences,Asan Medical Center, ²Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network,Korea Brain Research Institute

Summary

本协议描述了传输电子显微镜所需的各种技术, 包括阴性染色, 超薄切片的详细结构, 和免疫金标签, 以确定特定的蛋白质在体的位置。

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