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Biology
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优化低温电子断层扫描数据采集策略
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
Please note that all translations are automatically generated.
Click here for the English version.
优化低温电子断层扫描数据采集策略
DOI:
10.3791/62383-v
•
08:16 min
•
March 19, 2021
•
Felix Weis
,
Wim J. H. Hagen
,
Martin Schorb
,
Simone Mattei
3
1
Structural and Computational Biology Unit
,
European Molecular Biology Laboratory
,
2
Electron Microscopy Core Facility
,
European Molecular Biology Laboratory
,
3
Imaging Centre
,
European Molecular Biology Laboratory
Chapters
00:05
Introduction
00:38
Selecting the Targets
01:31
Generate Virtual Maps
02:07
Microscope Tuning
02:43
Set up Navigator
04:05
Set the Focus/Track Positions
05:00
Set up Additional Scripts
05:27
Run
06:19
Results: Optimization of Cryogenic Electron Tomography Data Acquisition
07:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
低温电子断层扫描对大规模数据收集的需求不断增加,这需要高通量图像采集程序。这里描述的是一个协议,旨在实现高级获取策略的最新发展,旨在最大限度地提高断层数据收集的时间效率和吞吐量。
Tags
Cryogenic Electron Tomography
Data Acquisition
SerialEM
Virtual Anchor Maps
Coma-free Alignment
Astigmatism Correction
Tilt Series Acquisition
Preview Maps
Focus/track Distance
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