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Journal / Biology / 体外sumo 法研究相扑 E3 连接活动
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体外sumo 法研究相扑 E3 连接活动
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体外sumo 法研究相扑 E3 连接活动

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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不像泛素酶, 很少有 E3 相扑酶被发现。这一修改的体外sumo 协议能够识别新的相扑 E3 酶由一个体外重建化验。

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分子生物学 问题 131,体外sumo K-bZIP 相扑 E3 连接 p53 后平移修饰 Ubc9
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