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Retrograd nevroanatomi sporing av Phrenic Motor Neurons i mus
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
DOI:

09:05 min

February 22, 2018

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Chapters

  • 00:05Title
  • 01:23Preparations
  • 02:22Intrapleural Injections Using Transdiaphragmatic Approach
  • 04:48Intrapleural Injection using Transthoracic Approach and Post-operative Care
  • 06:38Results: Labeling Neurons with CTB after a C4 Spinal Contusion
  • 08:01Conclusion

Summary

Automatic Translation

Her beskriver vi en protokoll for å identifisere phrenic motor neurons i mus etter intrapleural levering av fluorophore konjugert kolera gift delenhet beta. To teknikker sammenlignes for å injisere pleural hulrommet: transdiaphragmatic versus transthoracic tilnærminger.

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