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Chapters
- 00:05Title
- 01:23Preparations
- 02:22Intrapleural Injections Using Transdiaphragmatic Approach
- 04:48Intrapleural Injection using Transthoracic Approach and Post-operative Care
- 06:38Results: Labeling Neurons with CTB after a C4 Spinal Contusion
- 08:01Conclusion
Aqui, descrevemos um protocolo para identificar frênico neurônios em ratos após entrega intrapleural de fluoróforo conjugado beta de subunidade de toxina de cólera. Duas técnicas são comparadas para injetar a cavidade pleural: transdiaphragmatic versus transtorácicos abordagens.
