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Immunology and Infection

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JoVE Journal Immunology and Infection
Berigelse af bakteriel lipoproteiner og forberedelse af N-terminale Lipopeptides for Strukturbestemmelse af massespektrometri
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Berigelse af bakteriel lipoproteiner og forberedelse af N-terminale Lipopeptides for Strukturbestemmelse af massespektrometri

Article DOI: 10.3791/56842-v • 10:59 min • May 21st, 2018
May 21st, 2018 •
1, 1
1Department of Biochemistry and Molecular Biology, Pennsylvania State University

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Berigelse af bakteriel lipoproteiner ved hjælp af en nonionisk overfladeaktivt stof fase partitionering metode er beskrevet til direkte brug i TLR assays eller andre programmer. Yderligere skridt er detaljeret at forberede N-terminale tryptic lipopeptides for strukturel karakterisering af massespektrometri.

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Immunologi og infektion spørgsmålet 135 Lipoprotein bakterier Triton X-114 Toll-lignende receptor nitrocellulose lipopeptid MALDI-TOF N-terminus acylation struktur
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