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Journal / Immunology and Infection / Berigelse af indfødte og rekombinante…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Berigelse af indfødte og rekombinante ekstracellulære vesikler af mykobakterier
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Berigelse af indfødte og rekombinante ekstracellulære vesikler af mykobakterier

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Denne protokol beskriver berigelsen af indfødte mykobakterielle ekstracellulære vesikler (mEV'er) fra axeniske kulturer af Mycobacterium smegmatis (Msm), og hvordan mCherry (en rød fluorescerende reporter) -indeholdende rekombinante MsmEV'er kan designes og beriges. Endelig verificerer den den nye tilgang med berigelse af MsmEV'er, der indeholder EsxA-proteinet fra Mycobacterium tuberculosis.

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Immunologi og infektion udgave 202 Ekstracellulære vesikler rekombinante vesikler Esat-6 mykobakterier ExsA mCherry Cfp-29 bakterier
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