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Developmental Biology
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人胎脑神经干细胞 Zika 病毒感染的免疫细胞化学分析
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Developmental Biology
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JoVE Journal
Developmental Biology
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
Please note that all translations are automatically generated.
Click here for the English version.
人胎脑神经干细胞 Zika 病毒感染的免疫细胞化学分析
DOI:
10.3791/56917-v
•
10:27 min
•
February 05, 2018
•
Erica L. McGrath
2
,
Junling Gao
,
Ping Wu
3
1
Department of Neuroscience and Cell Biology
,
University of Texas Medical Branch
,
2
Department of Neurology
,
Johns Hopkins University
,
3
Beijing Institute for Brain Disorders
,
Capital Medical University
Chapters
00:04
Title
00:57
hNSC Passage
04:50
Plating Adherent hNSC for Validation
06:02
Zika Virus Infection
07:37
Results: Human Fetal Brain Neural Stem Cell Infection with Zika Virus
09:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文详细介绍了在培养人类胎儿脑神经干细胞的方法, 以及如何将它们区分成各种神经元亚型和星形胶质细胞, 重点是利用神经干细胞研究 Zika 病毒感染。
Tags
Zika Virus
Human Fetal Brain Neural Stem Cells
Immunocytochemical Analysis
In Vitro
Neural Stem Cell Differentiation
Innate Immune Response
Translational Application
Neurological Deficits
Cell Culture
Cell Passage
Trypsin
DNase
Centrifugation
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