9,077 Views
•
09:47 min
July 17, 2018
DOI:
10.3791/57197-v
このプロトコルでは、シアノ バクテリアの一時的な DNA の締固めを可視化する方法について説明します。同期の栽培、蛍光顕微鏡、急速凍結、高によって監視電圧低温電子断層撮影法を使用します。これらの方法のためのプロトコルを提案し、, 将来のアプリケーションや開発を議論しました。
09:59
Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
Related Videos
79085 Views
11:33
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
10855 Views
09:06
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
4184 Views
14:23
Electron Cryotomography of Bacterial Cells
25340 Views
09:12
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
6868 Views
10:39
Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
29710 Views
N/A
Simplified Volumetric Models as an Effective Strategy for Segmenting Actin Networks in Cryo-Electron Tomograms
208 Views
08:47
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
3786 Views
08:55
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
4722 Views
08:37
Visualization of Organelles In Situ by Cryo-STEM Tomography
1955 Views
Read Article
Cite this Article
Murata, K., Kaneko, Y. Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography. J. Vis. Exp. (137), e57197, doi:10.3791/57197 (2018).
Download .ris file
Copy
Share Video
.