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JoVE Journal Genetics
Gene targeting mutagenesi casuale per selezionare eterocromatina-destabilizzare Proteasome mutanti in lievito di fissione
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Gene targeting mutagenesi casuale per selezionare eterocromatina-destabilizzare Proteasome mutanti in lievito di fissione

Article DOI: 10.3791/57499 • 07:18 min
May 15th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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May 15th, 2018

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Questo articolo descrive una metodologia dettagliata per mutagenesi casuale di un determinato gene in lievito di fissione. Ad esempio, noi target rpt4 +, che codifica per una subunità del proteasoma 19S e schermo per le mutazioni che destabilizzare eterocromatina.

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