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Journal / Genetics / Biotin tabanlı açılan tahlil için Doğrula mRNA…
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JoVE Journal Genetics
Biotin tabanlı açılan tahlil için Doğrula mRNA hedefleri hücresel miRNAs
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Biotin tabanlı açılan tahlil için Doğrula mRNA hedefleri hücresel miRNAs

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Bu rapor hücresel miRNAs mRNA hedefleri doğrulamak için hızlı ve güvenilir bir yöntem açıklanır. Sentetik biotinylated miRNA kilitli nükleik asit LNA tabanlı yakalamaya taklit eden yöntemi kullanır mRNA hedef. Daha sonra manyetik boncuklar streptavidin kaplı açılan için istihdam edilmektedir hedef mRNA qPCR polimeraz zincir reaksiyonu tarafından miktar için.

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Genetik sayı: 136 miRNA mRNA LNA biotinylation PCR 3' UTR
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