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JoVE Journal
Genetics

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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
 

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Article DOI: 10.3791/58089-v 07:56 min November 16th, 2018
November 16th, 2018

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Summary

This protocol describes the transient genetic engineering of dental stem cells extracted from the human dental follicle. The applied non-viral modification strategy may become a basis for the improvement of therapeutic stem cell products.

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Tags

Isolation Characterization MicroRNA-based Genetic Modification Human Dental Follicle Stem Cells Regenerative Medicine Therapeutic Properties MicroRNA Introduction Stable Genome Integration Enzymatic Digestion Collagenase Type I Dispase II Basal Medium Antibiotic Agent Sterile Petri Dish PBSPSG Solution Extracted Tooth Follicle Sterile Scalpel Minced Tissue Conical Centrifuge Tube
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