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JoVE Journal Biochemistry
Medição da concentração de íons na camada limite Unstirred com pipeta aberto Patch-Clamp
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Journal (Biochemistry)
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Journal (Biochemistry)
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
 
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Medição da concentração de íons na camada limite Unstirred com pipeta aberto Patch-Clamp: implicações no controle de canais iônicos por fluido fluem

Article DOI: 10.3791/58228-v • 05:42 min • January 7th, 2019
January 7th, 2019 •
*1, *2, 1, 1, 3, 1
1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, 2Department of Emergency Medical Services, Eulji University, 3Department of Mechanical Engineering, Sungkyunkwan University
* These authors contributed equally

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Canais de íon Mechanosensitive muitas vezes são estudados em termos de sensibilidade de fluxo fluido/cisalhamento força com gravação de remendo-braçadeira. No entanto, dependendo do protocolo experimental, os resultados no fluxo fluidos-regulamentos de canais iônicos pode ser errôneo. Aqui, nós fornecemos métodos de prevenção e corrigir esses erros com uma base teórica.

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Bioquímica edição 143 fluxo de fluido cisalhamento força camada unstirred remendo-braçadeira eletrodo de referência Ag/AgCl potencial de junção de metal líquido convecção canal iônico
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