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Journal / Biochemistry / 高通量荧光素酶法评价单周转蛋白酶 PCSK9…
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Biochemistry

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JoVE Journal Biochemistry
高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解
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Journal (Biochemistry)
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Journal (Biochemistry)
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高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解

Article DOI: 10.3791/58265-v • 08:15 min • August 28th, 2018
August 28th, 2018 •
1, 2, 2
1Division of Cardiology, Department of Medicine, Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California San Francisco

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本协议提出了一种评估细胞内一种内在的低活性、单周转蛋白酶的蛋白水解活性的方法。具体来说, 该方法用于评价 PCSK9 的蛋白水解活性, 这是脂代谢的关键驱动力, 其蛋白水解活性是其最终胆固醇功能所必需的。

Tags

生物化学 问题 138 Proprotein convertase 枯草杆菌/科信型 9 蛋白酶 基底特异性 高通量测定 荧光素酶 低密度脂蛋白 低密度脂蛋白受体 单核苷酸多态性 药物筛选
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