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Journal
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Biochemistry
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使用光子芯片的高通量总内反射荧光和直接随机光学重建显微镜
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal
Biochemistry
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Please note that all translations are automatically generated.
Click here for the English version.
使用光子芯片的高通量总内反射荧光和直接随机光学重建显微镜
DOI:
10.3791/60378-v
•
14:09 min
•
November 16, 2019
•
David André Coucheron
,
Øystein Ivar Helle
,
Cristina Ionica Øie
,
Jean-Claude Tinguely
,
Balpreet Singh Ahluwalia
1
Department of Physics and Technology
,
UiT The Arctic University of Norway
,
2
Vascular Biology Research Group, Department of Medical Biology
,
UiT The Arctic University of Norway
Chapters
00:01
Title
01:13
Waveguide Cleaning
02:05
Sample Chamber Preparation
02:41
Fluorescent Labelling
04:12
Setup Components
04:56
Waveguide Coupling
07:26
Diffraction Limited Imaging
08:42
dSTORM Imaging
09:35
dSTORM Image Reconstruction
10:42
Results
12:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
基于芯片的超分辨率光学显微镜是荧光显微镜的一种新方法,具有成本效益和吞吐量方面的优势。此处显示了用于 TIRF 显微镜和基于定位的超分辨率显微镜的芯片制备和成像协议。
Tags
Photonic Chip
TIRF Microscopy
DSTORM
Evanescent Wave
High-throughput Imaging
Waveguide
PDMS
Sample Preparation
Cell Seeding
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