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JoVE Journal Developmental Biology
Analyse der Epididymal Protein-Synthese und Sekretion
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Analyse der Epididymal Protein-Synthese und Sekretion

Article DOI: 10.3791/58308-v • 10:23 min • August 25th, 2018
August 25th, 2018 •
1,2, 3, 1,2, 2,4, 1,2
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

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Hier berichten wir über die Immunfluoreszenz-Lokalisierung von Dynamin, die Protokolle für den Nachweis von Proteinen in Paraffin-eingebetteten Maus epididymal Abschnitten und derjenigen einer epididymal immortalisierte Zelllinie (mECap18) zu illustrieren. Wir beschreiben auch die Protokolle für die Isolierung von sekretorischen Proteine aus epididymal Flüssigkeit und konditionierten Zelle Medien.

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Entwicklungsbiologie Ausgabe 138 Dynamin Nebenhoden Epididymosome exosom Immunfluoreszenz Protein Sekretion Sperma spermienreifung
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