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JoVE Journal Neuroscience
Gelijktijdige Cryosectioning van meerdere knaagdier hersenen
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Gelijktijdige Cryosectioning van meerdere knaagdier hersenen

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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Hier presenteren we een protocol om te bevriezen en afdeling hersenweefsel van meerdere dieren als een tijdbesparende alternatief voor één hersenen verwerken. Dit vermindert kleuring variabiliteit tijdens immunohistochemistry en vermindert de tijd cryosectioning en beeldvorming.

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Neurowetenschappen kwestie 139 neurowetenschap histologie immunohistochemistry knaagdier cryoprotection cryosectioning cryostaat Neuropathologie
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