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Journal / Genetics / Um protocolo personalizáveis para String Assembly …
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Genetics

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JoVE Journal Genetics
Um protocolo personalizáveis para String Assembly gRNA clonagem (STAgR)
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Um protocolo personalizáveis para String Assembly gRNA clonagem (STAgR)

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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Aqui, apresentamos a sequência montagem gRNA clonagem (STAgR), um método facilmente multiplexar gRNA vetores para CRISPR/Cas9 aproxima-se. STAgR faz a multiplexação de gRNA simples, eficiente e personalizável.

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Genética questão 142 CRISPR Cas9 dCas9 gRNA clonagem gRNA multiplexação genoma edição edição de transcriptoma
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