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Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
JoVE Journal
Genetics
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JoVE Journal Genetics
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

DOI:
10.3791/63250-v

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06:18 min

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November 30, 2021

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, , ,
1Department of Archaeogenetics,Max Planck Institute for the Science of Human History, 2School of Human Evolution and Social Change,Arizona State University, 3Department of Archaeogenetics,Max Planck Institute for Evolutionary Anthropology

Chapters

  • 00:04Introduction
  • 00:38Sampling of the Cementum
  • 02:34Sampling of the Superior Vertebral Arch
  • 03:51Sampling of the Talus
  • 05:04Results: Recovery of Endogenous DNA from Anatomical Samples
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents a series of best practice protocols for the collection of bone powder from eight recommended anatomical sampling locations (specific locations on a given skeletal element) across five different skeletal elements from medieval individuals (radiocarbon dated to a period of ca. 1040-1400 CE, calibrated 2-sigma range).

Tags

Keywords: Ancient DNABone SamplingSkeletal ElementsDNA ExtractionForensic RemainsNon-human SpecimensDentinPetrous PyramidDental DrillBone PowderVertebral ArchSpinous ProcessCortical BoneDNA Sampling Protocols

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