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Genetics

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JoVE Journal Genetics
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Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Article DOI: 10.3791/63250-v • 06:18 min • November 30th, 2021
November 30th, 2021 •
1,2, 1,3, 1,3, 1,3
1Department of Archaeogenetics, Max Planck Institute for the Science of Human History, 2School of Human Evolution and Social Change, Arizona State University, 3Department of Archaeogenetics, Max Planck Institute for Evolutionary Anthropology

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Summary
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The protocol presents a series of best practice protocols for the collection of bone powder from eight recommended anatomical sampling locations (specific locations on a given skeletal element) across five different skeletal elements from medieval individuals (radiocarbon dated to a period of ca. 1040-1400 CE, calibrated 2-sigma range).

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Bone Sampling Protocols Ancient DNA Retrieval Best Practice Guidelines Downstream DNA Extraction Skeletal Elements Dentin Petrous Pyramid Sampling Methods Degraded Forensic Remains Clean Room UV Light-equipped PCR Hood Biosafety Cabinet Bone Powder Weighing Tray Dental Drill Circular Cutting Wheel Dentin Material LoBind Safe-Lock Tube Collected Mass Of Powder
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