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利用笼子分子胶作为可活性标记的活细胞中的贵宾的时空控制核移位
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JoVE Journal
Chemistry
Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
Please note that all translations are automatically generated.
Click here for the English version.
利用笼子分子胶作为可活性标记的活细胞中的贵宾的时空控制核移位
DOI:
10.3791/58631-v
•
10:10 min
•
January 17, 2019
•
Rina Mogaki
,
Kou Okuro
,
Akio Arisaka
,
Takuzo Aida
2
1
Department of Chemistry and Biotechnology, School of Engineering
,
University of Tokyo
,
2
Riken Center for Emergent Matter Science
Chapters
00:04
Title
00:29
Preparation of Guests with
Caged
Glue-R Tags
02:17
Preparation of Hep3B Cell Sample for Microscopic Observations
02:59
Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
04:53
Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
05:48
Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
07:04
Cell Viability Assay
08:20
Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
09:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了使用笼子分子胶标签的光触发核移位的客人在活细胞中的位置。该方法对现场选择性核靶向药物的输送具有广阔的应用前景。
Tags
Nuclear Translocation
Caged Molecular Glue
Photoactivatable Tags
Quantum Dot
Drug Delivery
HEP3B Cells
Fluorescent Dye
Endosomes
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