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JoVE Journal Biochemistry
Coloração fluorescente de prata das proteínas em gel de poliacrilamida
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Coloração fluorescente de prata das proteínas em gel de poliacrilamida

Article DOI: 10.3791/58669-v • 06:24 min • April 21st, 2019
April 21st, 2019 •
1, 1, 2, 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

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Aqui, descrevemos um protocolo detalhado descrevendo uma fluorescente nova técnica para detecção de proteínas totais em gel de poliacrilamida de coloração. O protocolo utiliza uma sonda de excitante prata íon-específicos de fluorescência, que detecta Ag+-complexos de proteínas e elimina determinadas limitações de manchas de prata cromogênica tradicionais.

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Bioquímica edição 146 coloração prata coloração fluorescente gel eletroforese em gel polyacrylamide do sulfato dodecyl de sódio (SDS-PAGE) induzida pela agregação de emissão (AIE) sensor de íon prata fluorogenic detecção proteomics
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