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Journal
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Immunology and Infection
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原代人细胞中寨卡病毒抗体依赖性增强的定量研究
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
Please note that all translations are automatically generated.
Click here for the English version.
原代人细胞中寨卡病毒抗体依赖性增强的定量研究
DOI:
10.3791/58691-v
•
07:53 min
•
January 18, 2019
•
Sultan Asad
2
,
Fabiana Feitosa-Suntheimer
2
,
Alexander Gold
2
,
Berlin Londono-Renteria
,
Tonya M. Colpitts
2
1
Department of Microbiology
,
Boston University School of Medicine
,
2
National Emerging Infectious Diseases Laborator
,
Boston University School of Medicine
,
3
Department of Entomology
,
Kansas State University
Chapters
00:04
Title
00:59
Cell Seeding and Infection Setup
02:46
RNA Extraction and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) Analysis
05:58
Results: Dengue Virus (DENV) Immune Sera Enhances Zika Virus (ZIKV) Infection in Primary Human Macrophages
06:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种方法来评估现有的免疫对登革热病毒感染的影响, 使用人血清, 原代人类细胞, 和感染定量定量定量定量定量定量定量定量定量定量定量定量定量的定量。
Tags
Antibody-dependent Enhancement
Zika Virus
Primary Human Cells
Dengue Virus
Pre-existing Immunity
Serum Samples
Virus Infection
Cell Culture
Immune Complexes
Cell Lysis
Quantification
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