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Journal / Biochemistry / Hurtig fluorescens-baserede karakterisering af…
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Biochemistry

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JoVE Journal Biochemistry
Hurtig fluorescens-baserede karakterisering af enkelt ekstracellulære vesikler i humant blod med nanopartikler-tracking analyse
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Hurtig fluorescens-baserede karakterisering af enkelt ekstracellulære vesikler i humant blod med nanopartikler-tracking analyse

Article DOI: 10.3791/58731-v • 09:16 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1
1Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University

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I denne protokol beskriver vi fuldstændig arbejdsgangen for hurtige isolering af ekstracellulære vesikler fra humant fuldblod og karakterisering af specifikke markører af fluorescens-baserede nanopartikel-tracking analyse. De præsenterede resultater viser en høj grad af reproducerbarhed og kan justeres til celle kultur analysere.

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Biokemi spørgsmålet 143 Exosomes nanovesicles partikel tracking analyse CD63 CD9 vimentin serum
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