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Journal
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Biochemistry
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一种用于蛋白质溶解活性的荧光肽裂解检测: 冠状病毒穗蛋白活化的应用
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
Please note that all translations are automatically generated.
Click here for the English version.
一种用于蛋白质溶解活性的荧光肽裂解检测: 冠状病毒穗蛋白活化的应用
DOI:
10.3791/58892-v
•
07:53 min
•
January 09, 2019
•
Javier A. Jaimes*
1,2
,
Jean K. Millet*
1,3
,
Monty E. Goldstein
4
,
Gary R. Whittaker
,
Marco R. Straus
1
Department of Microbiology and Immunology, College of Veterinary Medicine
,
Cornell University
,
2
Department of Microbiology, College of Agricultural and Life Sciences
,
Cornell University
,
3
Virologie et Immunologie Moléculaires, Domaine de Vilvert
,
INRA
,
4
Department of Cell Biology and Molecular Genetics
,
University of Maryland
Chapters
00:04
Title
00:31
Designing and Preparing the Peptides
02:06
Preparing the Fluorescence Plate Reader
03:11
Preparing the Assay
04:30
Data Analysis
05:37
Results: Mutations in the Cleavage Site Alter Activity of Furin and Cleavage Efficiency
07:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个氟原肽裂解检测, 允许快速筛选蛋白酶的蛋白溶解活性肽代表病毒融合肽的裂解部位。这种方法也可以用于蛋白质序列中的任何其他氨基酸母题, 以测试蛋白酶活性。
Tags
Fluorogenic Peptide Cleavage Assay
Proteolytic Activity
Coronavirus Spike Protein Activation
Proteolytic Modification
Protease Characterization
Viral Fusion
Peptide Design
FRET Pair
Fluorescence Plate Reader
Protease Activity Assay
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