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Journal
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Immunology and Infection
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小鼠肺损伤的诱导,通过博霉素内切注射
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin
Please note that all translations are automatically generated.
Click here for the English version.
小鼠肺损伤的诱导,通过博霉素内切注射
DOI:
10.3791/58922-v
•
06:52 min
•
April 30, 2019
•
Fiorenza Orlando*
1
,
Chiara Paolini*
2
,
Silvia Agarbati
,
Cecilia Tonnini
,
Antonella Grieco
,
Chiara Capelli
,
Martino Introna
,
Mauro Provinciali
,
Armando Gabrielli
,
Gianluca Moroncini
1
Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico
,
Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA)
,
2
Dipartimento di Scienze Cliniche e Molecolari
,
Università Politecnica delle Marche
,
3
UOS Centro di Terapia Cellulare “G. Lanzani”
,
Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII
Chapters
00:04
Title
00:56
Endotracheal Injection of Bleomycin
03:12
Tail Vein Infusion of Human Umbilical Cord Mesenchymal Stromal Cells (hUC-MSC)
04:53
Results: Assessment of Lung Injury after Bleomycin Injection and Repair after hUC-MSC Injection
06:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种有效的方法,以研究静脉注射人类中发性血质细胞的抗纤维化活性,从整个脐带获得后,通过在C57BL的内切治疗注射注射肺损伤/6 小鼠。该协议可以很容易地扩展到其他疗法的临床前测试。
Tags
Mouse Lung Injury
Bleomycin
Endotracheal Injection
Inflammation
Fibrosis
Anti-inflammatory
Anti-fibrotic Therapies
Trachea
Surgical Procedure
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