Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

This protocol uses three approaches to determine if cigarette smoke affects pseudomonas bacterial load in lung epithelial cells. This method can be expanded to endothelial cells or other cell taps. Preparation of the cigarette smoke extraction, bacterial infection and the determination with a bacterial load are well described in detail in this video.

This procedure is very easy to follow for the new users. Draw 10 milliliters of serum-free cell culture medium into a 60 milliliter syringe. Attach the narrow end of a trim to one milliliter pipette tip to the nozzle of the syringe, as an adapter to hold the cigarette.