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Immunology and Infection

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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

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Immunology and Infection

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JoVE Journal Immunology and Infection

Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells

DOI:

10.3791/61163-v

•

09:15 min

•

May 11, 2020

•

Tiao Li, Chen Long, Kristen V. Fanning, Chunbin Zou

¹Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine,University of Pittsburgh School of Medicine

Chapters

00:04Introduction
00:42Cigarette Smoking Extract (CSE) Preparation
01:39Pseudomonas Culture
02:16Cell Culture and CSE Treatment
03:33Bacterial Infection and Gentamycin Treatment
04:24Determination of Bacterial Concentration Using the Drop Plate Method
04:55RT-qPCR Detection of Bacterial 16S rRNA
07:00Results: CSE-Treatment Increases Bacterial Load
08:23Conclusion

Summary

Automatic Translation

Described here is a protocol to study how cigarette smoke extract affects bacterial colonization in lung epithelial cells.

Tags

Keywords: Cigarette Smoke Pseudomonas Lung Epithelial Cells Bacterial Load Cell Culture Smoke Extraction Bacterial Infection Cell Counting Cell Suspension Cell Preparation

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