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Immunology and Infection

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JoVE Journal Immunology and Infection
Merkel celle Polyomavirus infeksjon og gjenkjenning
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Merkel celle Polyomavirus infeksjon og gjenkjenning

Article DOI: 10.3791/58950-v • 13:45 min • February 7th, 2019
February 7th, 2019 •
*1, *1, 2, 1
1Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 2Laboratory of Cellular Oncology, National Cancer Institute
* These authors contributed equally

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Her presenterer vi en protokoll for å infisere primære menneskelige dermal fibroblast med MCPyV. Protokollen inneholder isolering av dermal fibroblaster, utarbeidelse av MCPyV virions, virusinfeksjon, immunofluorescence flekker og fluorescens i situ hybridisering. Denne protokollen kan utvides for karakteriserer MCPyV vert interaksjoner og oppdage andre celletyper infectable ved MCPyV.

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Immunologi og infeksjon problemet 144 Merkel celle polyomavirus isolasjon dermal fibroblaster infeksjon collagenase IV CHIR99021 immunofluorescent flekk fluorescens i situ hybridisering i situ DNA-hybridisering kjedereaksjon transkripsjon replikering
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