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JoVE Journal Genetics
Uso en Vitro y en las células la forma para investigar la molécula pequeña inducida por cambios estructurales Pre-mRNA
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Uso en Vitro y en las células la forma para investigar la molécula pequeña inducida por cambios estructurales Pre-mRNA

Article DOI: 10.3791/59021-v • 11:58 min • January 30th, 2019
January 30th, 2019 •
1, 2, 1
1Calibr, The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Protocolos detallados para ambos acilación in vitro y en células selectivo 2'-hidroxilo por experimentos de extensión (forma) de cartilla para determinar la estructura secundaria de las secuencias de interés en presencia de una pequeña molécula de RNA-dirigido a pre-mRNA son presentados en este artículo.

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Este mes en JoVE número 143 moléculas pequeñas dirigidas a la RNA RNA en vitro forma la estructura forma atrofia muscular espinal RG-7916 en células
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