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直接注射慢病毒载体突出大鼠脊髓中的多种运动途径
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Neuroscience
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JoVE Journal Neuroscience
Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
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直接注射慢病毒载体突出大鼠脊髓中的多种运动途径

DOI:
10.3791/59160-v

•

07:57 min

•

March 15, 2019

•

, , , ,
1Shriner’s Pediatric Research Center,Temple University

Chapters

  • 00:04Title
  • 00:45Surgical Preparation
  • 02:13Vertebral Column Exposure
  • 03:44Laminectomy
  • 04:36Virus Loading
  • 05:48Virus Injection and Post-Operative Care
  • 06:42Results: Representative Transduction of Neurons in the Spinal Cord and Brainstem
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

该协议演示了在大鼠脊髓组织中注射一种具有可追溯性的可转移性病毒载体。载体在突触处被占据, 并被输送到目标神经元的细胞体。该模型适用于重要脊柱通路的逆行追踪或基因治疗应用中的靶向细胞。

Tags

Lentiviral VectorSpinal CordMotor PathwaysViral VectorGenetic MaterialNeural CircuitsDirect InjectionL1 To L4 Spinal LevelsMicromanipulatorAnesthetized RatSkin IncisionSpinous ProcessesRat Tooth Forceps

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