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Journal / Biochemistry / Uttrykk, Solubilization og rensing av eukaryote…
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Biochemistry

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JoVE Journal Biochemistry
Uttrykk, Solubilization og rensing av eukaryote Borate transportører
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Uttrykk, Solubilization og rensing av eukaryote Borate transportører

Article DOI: 10.3791/59166-v • 08:55 min • March 7th, 2019
March 7th, 2019 •
1, 1, 1
1Department of Biology, Davidson College

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Her presenterer vi en protokoll for å uttrykke solubilize og rense flere eukaryote borate transportører med homologi til SLC4 transporter familien bruke gjær. Vi beskriver også en kjemisk cross-linking analysen for å vurdere renset homomeric proteiner for multimeric montering. Disse protokollene kan tilpasses for andre utfordrende membran proteiner.

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Biokjemi problemet 145 biokjemi strukturell biologi membran proteiner protein rensing Bor1 transportører bikarbonat transportører borate transportører SLC SLC4 Band 3 AE1
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