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Journal / Biochemistry / PCR Mutagenesis, Kloning, Uttrykk, Rask Protein…
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Biochemistry

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JoVE Journal Biochemistry
PCR Mutagenesis, Kloning, Uttrykk, Rask Protein Rensing Protokoller og Krystallisering av Wild Type og Mutant Former for Tryptophan Synthase
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Journal (Biochemistry)
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Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
Journal (Biochemistry)
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PCR Mutagenesis, Kloning, Uttrykk, Rask Protein Rensing Protokoller og Krystallisering av Wild Type og Mutant Former for Tryptophan Synthase

Article DOI: 10.3791/61839-v • 09:31 min • September 26th, 2020
September 26th, 2020 •
1, 2, 1, 2
1Department of Chemistry, University of California-Riverside, 2Department of Biochemistry, University of California-Riverside

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Denne artikkelen presenterer en rekke påfølgende metoder for uttrykk og rensing av Salmonella typhimurium tryptofan synthase comp denne protokollen et raskt system for å rense proteinkomplekset på en dag. Dekkede metoder er stedsstyrt mutagenese, proteinuttrykk i Escherichia coli, affinitetskromatografi, gelfiltreringskromatografi og krystallisering.

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Biokjemi Utgave 163 Tryptofan syntase rekombinant protein mutantprotein proteinuttrykk proteinrensing ammoniumsulfatutfelling størrelseseksklusjonskromatografi proteinkrystallisering
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