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Chapters
Summary
A rapidly-deployable, off-grid laboratory has been designed and built for remote, resource-constrained global settings. The features and critical aspects of the logistically-enhanced, expandable, multifunctional laboratory modules are explored. A checklist for a basic laboratory workflow and a protocol for a respiratory viral diagnostic test are developed and presented.
Tags
Remote Laboratory Management Respiratory Virus Diagnostics Epidemics Zika Virus Ebola Virus Coordinated Response Global Scale Innovative Laboratory Unit Resource-constrained Areas Intra-modal Expandable Mobile Shipping Container Laboratories BSL-2 Approach BSL-3 Approach Respiratory Influenza Virus Diagnosis RCT-PCR BSL-2 Safety Requirements Personal Protective Equipment Handwashing Gloves Decontamination Bleach 70% Ethanol Toxic Fumes Designated Waste Bin Sample Swabs Laboratory Facility Pass-through Window
PLAYLIST
Behavior
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Research • BehaviorNovel Object Recognition Test for the Investigation of Learning and Memory in Mice -
Research • BehaviorNovel Object Recognition and Object Location Behavioral Testing in Mice on a Budget -
Research • BehaviorAssessment and Evaluation of the High Risk Neonate: The NICU Network Neurobehavioral Scale
Biochemistry
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Research • BiochemistryA Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation -
Research • BiochemistryPurification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion -
Research • BiochemistryCharacterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Bioengineering
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Research • BioengineeringOperation of a Benchtop Bioreactor -
Research • BioengineeringStandardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro -
Research • BioengineeringElectric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
Biology
Cancer Research
Chemistry
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Research • ChemistryConducting Miller-Urey Experiments -
Research • ChemistryUtilization of Stop-flow Micro-tubing Reactors for the Development of Organic Transformations -
Research • ChemistryMatrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Developmental Biology
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Research • Developmental BiologyThawing, Culturing, and Cryopreserving Drosophila Cell Lines -
Research • Developmental BiologyIsolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta -
Research • Developmental BiologyDifferentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Engineering
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Research • EngineeringA Polymer-based Piezoelectric Vibration Energy Harvester with a 3D Meshed-Core Structure -
Research • EngineeringThree-electrode Coin Cell Preparation and Electrodeposition Analytics for Lithium-ion Batteries -
Research • EngineeringCarrier Lifetime Measurements in Semiconductors through the Microwave Photoconductivity Decay Method
Environment
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Research • EnvironmentProtocol for Microplastics Sampling on the Sea Surface and Sample Analysis -
Research • EnvironmentIsolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments -
Research • EnvironmentSampling Soils in a Heterogeneous Research Plot
Genetics
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Research • GeneticsCRISPR Guide RNA Cloning for Mammalian Systems -
Research • GeneticsPromoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions -
Research • GeneticsTargeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Immunology and Infection
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Research • Immunology and InfectionProduction of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting -
Research • Immunology and InfectionA Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells -
Research • Immunology and InfectionViral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems
Medicine
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Research • MedicineA Case Series of Successful Abdominal Closure Utilizing a Novel Technique Combining a Mechanical Closure System with a Biologic Xenograft that Accelerates Wound Healing -
Research • MedicineRemote Laboratory Management: Respiratory Virus Diagnostics -
Research • MedicineSingle-stage Dynamic Reanimation of the Smile in Irreversible Facial Paralysis by Free Functional Muscle Transfer
Neuroscience
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Research • NeuroscienceWhole Animal Perfusion Fixation for Rodents -
Research • NeuroscienceCompartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips -
Research • NeuroscienceUse of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
