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小鼠透明血管的评估与表征
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JoVE Journal Medicine
Assessment and Characterization of Hyaloid Vessels in Mice
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小鼠透明血管的评估与表征

DOI:
10.3791/59222-v

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08:22 min

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May 15, 2019

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, , ,
1Department of Ophthalmology, Boston Children’s Hospital,Harvard Medical School, 2The Manton Center for Orphan Disease Research, Boston Children’s Hospital,Harvard Medical School

Chapters

  • 00:04Title
  • 01:31In Vivo Hyaloid Vessel Imaging
  • 02:23Hyaloid Vessel Harvest for Ex Vivo Visualization
  • 04:50Hyaloid Vessel Flat Mounting and Ex Vivo Imaging
  • 05:57Results: Representative In Vivo and Ex Vivo Visualization
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了体内和体外方法,以完全可视化和表征透明血管,小鼠眼睛血管回归的模型,使用光学相干断层扫描和荧光素血管造影进行活成像和外体成像隔离和随后的透明质的扁平安装,用于定量分析。

Tags

Hyaloid VesselsVascular RegressionPersistent Fetal VasculatureAngiogenesisOptical Coherence TomographyFundus Fluorescein AngiographyMicrosurgery ForcepsVitreous BodyEyeball Dissection

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