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In vivo Nöromimarlık çalışmaları için tasarlanan nöronal öncülerin intraventriküler nakli
JoVE Journal
Genetics
Author Produced
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JoVE Journal Genetics
Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
DOI:

15:00 min

May 11, 2019

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Chapters

  • 00:00Title
  • 00:42The Procedure
  • 01:41Preparation of the Green Pool
  • 02:09Engineering the Green Sub-pools
  • 03:19Preparing Borosilicate Needles
  • 04:17Disinfecting the Hood and Preparing Cell Tracer
  • 04:48Assembling the Injection Tube
  • 05:20Mixing “Test” and “Control” Green Sub-Pools
  • 06:24Intraventricular Transplantation
  • 09:11Histology and Immunoflourescence
  • 10:09Morphometry
  • 12:52Results: Neuroarchitectural Analysis of Neurons Overexpressing a Neocortical Patterning Gene
  • 14:17Conclusion

Summary

Automatic Translation

Nöronal öncülerin in vitro lentiviral mühendisliğine dayanarak, vahşi tip beyinleri ve "test" ve "kontrol" türevlerinin eş-transplantasyonu, bu yöntem, neokortikal in vivo gen kontrolünde doğru modelleme sağlar basit ve ekonomik bir şekilde nöron morfoloji.

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