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人类舱内单聚物研究病原体、共脉和宿主肠道上皮细胞之间的相互作用
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
DOI:

07:20 min

April 09, 2019

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Chapters

  • 00:04Title
  • 01:22Isolation and Dissociation of Enteroids/Colonoids from 3D Culture
  • 02:39Plating the Enteroid/Colonoid Suspension
  • 04:10Fixation to Preserve and Immunostain Mucus
  • 05:16Results: Establishment and Evaluation of Human Enteroid/Colonoid Monolayers
  • 06:51Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个培养人类肠体或类团类单层具有完整的屏障功能的方案, 以研究宿主上皮微生物在细胞和生化水平上的相互作用。

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