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Immunology and Infection
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Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

DOI:
10.3791/63773-v

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07:28 min

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June 08, 2022

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, ,
1Department of Microbiology,The Ohio State University, 2Department of Microbial Infection and Immunity,The Ohio State University

Chapters

  • 00:04Introduction
  • 00:54Preparation of In Vitro Biofilm
  • 02:34Measurement of ROS Production by Neutrophils
  • 04:14Imaging the Biofilm-Neutrophil Interactions
  • 05:31Results: Assessing Neutrophils and Biofilms Interactions via Standardized In Vitro Assays
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the study of neutrophil-biofilm interactions. Staphylococcus aureus biofilms are established in vitro and incubated with peripheral blood-derived human neutrophils. The oxidative burst response from neutrophils is quantified, and the neutrophil localization within the biofilm is determined by microscopy.

Tags

Keywords: NeutrophilsStaphylococcus AureusBiofilmsIn Vitro AssaysOpsonizationLuminolSerumPLLMEM-alphaHBSSBiofilm FormationBiofilm QuantificationStandardized Protocols

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