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Journal / Biochemistry / Single-molekyl tracking mikroskopi-ett verktyg…
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Biochemistry

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JoVE Journal Biochemistry
Single-molekyl tracking mikroskopi-ett verktyg för att bestämma Diffusiva stater cytosoliska molekyler
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Single-molekyl tracking mikroskopi-ett verktyg för att bestämma Diffusiva stater cytosoliska molekyler

Article DOI: 10.3791/59387-v • 10:20 min • September 5th, 2019
September 5th, 2019 •
1, 1,2
1Department of Chemistry, University of Virginia, 2Department of Molecular Physiology & Biological Physics, University of Virginia School of Medicine

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3D Single-molekylen lokalisering mikroskopi utnyttjas för att söka de rumsliga positioner och rörelsebanor av överföras märkta proteiner i levande bakterieceller. Den experimentella och dataanalys protokoll som beskrivs häri avgör de utbredda diffusiva beteenden av cytosoliska proteiner baserade på poolade enda molekyl Trajectories.

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Biokemi fysikalisk kemi organismer bakterier kemi och material oorganisk organisk och fysikalisk kemi biovetenskap biovetenskap (allmän) matematisk och datavetenskap numerisk analys super-resolution Single-molekyl fluorescens spårning diffusion Live-cell
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