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下颌骨和腮腺表型模型对组织型培养的放射治疗是机体活力特征的关键
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JoVE Journal Medicine
Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics
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下颌骨和腮腺表型模型对组织型培养的放射治疗是机体活力特征的关键

DOI:
10.3791/59484-v

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07:38 min

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May 17, 2019

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, , , ,
1Department of Nutritional Sciences,University of Arizona, 2Cancer Biology Graduate Interdisciplinary Program,University of Arizona, 3Department of Chemical Engineering,University of Arizona

Chapters

  • 00:04Title
  • 01:02Vibratome Preparation
  • 01:50Tissue Sample Preparation and Sectioning
  • 03:19Salivary Gland Section Culture
  • 04:01Salivary Gland Section Irradiation and Antibody Staining
  • 05:17Results: Representative Characteristics of In Vivo Salivary Gland Section 2D Cultures
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

利用三维有机型培养物来可视化唾液腺的形态和功能标记, 可以为辐射后组织损伤的机制提供新的见解。这里描述的是一个协议的部分, 培养, 辐照, 染色, 和图像50-90 微米厚的唾液腺部分之前和之后的电离辐射照射。

Tags

Organotypic CultureSubmandibular Salivary GlandParotid Salivary GlandRadiation TreatmentEx VivoVibratomeAgarose EmbeddingCell-cell InteractionsTherapeutic ScreeningSalivary Gland DamageRadiotherapyGene Manipulation

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